BIOTECHNOLOGICAL STUDIES ON SALINITY-TOLERANT MICROORGANISMS.

Halophilic and halo-tolerant bacteria are micro-organisms that living in hyper saline environments. They have many potential several fields life such as Industry agriculture. In this study a total of 17 isolates were obtained from the four ponds Qarun Lake on Nutrient Agar (NA) medium prepared using ponds' water. The 16S rRNA analysis showed our affiliated with groups, Firmicutes (47.06% isolates) include family Bacillaceae six different genera including Bacillus, Halobacillus, Lentibacillus, Oceanobacillus, Thalassobacillus Virgibacillus, Gammaproteobacteria (35.29% two Halomonas Salinivibrio, within Bacteriodetes group (11.76% one Alphaproteobacteria (5.88%). seems to be more dominant group, microorganisms various genera, especially Bacillus (37.5%) followed by Lentibacillus Virgibacillus (12.5% for each genera). 52.94% gram negative, 35.29% positive 11.76% variable. All catalase producers motile. Halotolerance assay observed only (11.76 %) able grow absence salt considered halotolerant, while fifteen (88.24%) NA media concentrations halophilic bacteria. Among them, thirteen (76.47%) 7% NaCL. grew 12.5% NaCl; nine iso-lates (52.94%) 20% salinity, three (17.65%) appeared concentration 22% extremely tested plant growth promoting factors. results all can produce IAA, 47.06% fix Nitrogen 47.06 % ability phosphate solubilization. Concerning hydrolytic enzymes activi-ties isolates, it was found 58.82% 76.47% pro-duced lipase olive oil tween80 substrate respectively. It also esterase production same percentage casease production. Gelatinase excereted 58.82%, chitinase produced 70.59% isolates. Five screened biosurfactants other bioactive compounds These namly QSLA1, QSLA7, QSLA14, QSLA16 QSLA17 which similar to, sp.RS-17, Salinivibrio sp. pr6, sp.strain BCHS25, Uncultured bacterium clone QAMU23 sp.strain6 GenBank database, them had NRPs genes encoding lipopeptide synthetase HPLC confirmed presence as, surfactin, fengycin mycosubtilin. culture QSLA1and QSLA7 analysed Gas chromatography–mass spectrometry (GC-MS) antifungal, antimicrobial antitumer metabolites Desulphosinigrin, Undeca-2,4,6,8,10-pentaenal, 11-(2-furyl)-, oxime Strychane, 1-acetyl-20à-hydroxy-16-methylene. Accordingly, QSLA1 vitro antibacterial activity against Salmonella typhi Acinetobacter baumannii. Whereas, inhibit bacterial pathogens Staphylococcus aureus, Klebsiella pneumonia contaminated salty whey. regard, antifungal Alternaria solani Fusarium oxysporium f.sp. lycopersici, vivo siginificant effect lycopersici infecting tomato seedlings compared control therefore used among biological systems under conditions